Description
The system uses Illumina’s proven 2-channel Sequencing by Synthesis (SBS) chemistry, delivering accurate, high-quality results with a streamlined workflow. In addition to sequencing, the NextSeq 550 supports array-based assays such as genotyping and methylation profiling.
Key Technical Specifications:
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Flow Cell Options and Output:
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High Output Mode: Up to 400 million reads per run (2×150 bp), yielding approximately 120 Gb of data
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Mid Output Mode: Up to 130 million reads per run (2×150 bp), yielding approximately 39 Gb of data
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Read Lengths Supported:
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1×75 bp
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2×75 bp
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2×150 bp
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Run Times:
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2×150 bp High Output: ~29 hours
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2×75 bp High Output: ~18 hours
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2×150 bp Mid Output: ~26 hours
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Base Quality:
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75% of bases above Q30 for 2×150 bp runs
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80% of bases above Q30 for 2×75 bp runs
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Optics:
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Dual-wavelength LED excitation system with green and red detection channels
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2-channel SBS technology for faster imaging and fewer cycles per run
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Instrument Dimensions and Power:
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Size: 53.3 cm (W) × 63.5 cm (D) × 58.4 cm (H)
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Weight: ~83 kg (183 lb)
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Power: 100–120V, 15A or 220–240V, 10A
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Array Scanning:
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Integrated array scanning functionality for BeadChip-based assays
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Software & Analysis:
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Compatible with Local Run Manager and cloud-based BaseSpace Sequence Hub
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