Description
The nCounter Sprint Profiler is an all-in-one benchtop nCounter Analysis System that combines the function of the nCounter Pro Prep Station and Digital Analyzer into one instrument to produce gold-standard multiplex data and save bench space by fitting on a single laboratory bench.
Hybridized samples are loaded into a SPRINT cartridge, where a series of microfluidic chambers purifies and immobilizes the fluorescent Reporter Probes and readies them for imaging. Images are processed, and output files include gene names along with associated count numbers.
Specifications:
- Level of multiplexing: Up to 800 targets
- Recommended amount of starting material (Dependent on assay and sample type)
RNA: 1 ng-50 ng DNA: 5 ng-300 ng - Sample types supported: Total RNA, cell lysates in GITC, FFPE-derived total RNA, and PAXgene-lysed whole blood
- Reaction Volume: Up to 35 µl
- Limit of detection 0.5 fM spike-in control: 15 zeptomole spike-in control
- Fold change sensitivity: > 1.5-fold (if > 5 copies per cell) > 2-fold (if > 1 copy per cell)
- Spike correlation: R² ≥ 0.95
- Linear dynamic range: 6 x 105 total counts
- Throughput: 12 lanes per 6 hours
- Controls: Assay dependent
