Description
Filter-based multi-mode microplate reader
The FLUOstar Omega is a multi-mode microplate reader with three detection modes. It utilises an ultra-fast UV/vis spectrometer or filters for absorbance as well as highly sensitive filters for all other detection modes. The FLUOstar Omega is the ideal plate reader for life science applications.
Specifications:
Detection Modes:
Fluorescence intensity – incl. FRET
Luminescence (flash and glow) – incl. BRET
UV/Vis absorbance spectra
Measurement Modes:
Top and bottom reading
Endpoint and kinetic
Sequential multi excitation
Sequential multi emission
Simultaneous Dual Emission (FI and LUM)
Spectral scanning (absorbance)
Ratiometric measurements
Microplate Carrier: Robot compatible
Light Sources: High energy xenon flash lamp
Detectors: Low noise photomultiplier tube, CCD spectrometer
Wavelength Selection:
Optical filters: ex and em wheels for 8 filters each
UV/vis absorbance spectrometer: full spectra or 8 distinct wavelengths in < 1 sec/well
Spectral Range:
Filters
240 - 740 nm or 240 - 900 nm for FI
240 - 740 nm for LUM
Spectrometer: 220 - 1000 nm for ABS; wavelength precision: ≤ 0.5 nm
Sensitivity:
FI: <0.2 fmol/well FITC.
TRF: < 30 amol/well europium.
LUM: < 20 amol/well ATP.
ABS with spectrometer
Selectable spectral resolution: 1, 2, 5, and 10 nm.
OD range: 0 to 4 OD; photometric resolution: 0.001 OD.
Accuracy: < 1% at 2 OD.
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD.
Linearity: ≤ 0.8% at 2.0 OD.
ABS with filters
OD range: 0 to 4 OD
Reproducibility: ±0.010 OD for 0-2 OD range
Read times: Flying mode ( 1 flash ) 9 s (96, 16 s (384)
Shaking: Linear, orbital, and double-orbital with user-definable time and speed
Purge Gas Vent: System to inject an atmosphere or to pull a vacuum into the reader
Incubation:
+4 °C above ambient up to 45 °C or 65 °C
The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer
Software: Multi-user Reader Control and MARS Data Analysis Software included, FDA 21 CFR Part 11 compliant
Dimensions: Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg
Optical Filters: Excitation and emission filter wheels for 8 filters each
Optical Path Guides: Top: liquid-filled light guides, Bottom: fiber optics
Reagent Injection:
Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL)
Variable injection speed up to 420 µL/s
Up to four injection events per well
Reagent back flushing
Power Requirements: 100-240 V, 50/60 Hz, max. peak 300 VA
Power Consumption:
Instrument off: 0 W
Normal operating conditions: 25 W
Normal operating conditions, incubation set to 37°C: 60 W
