Description
The Agilent Seahorse XFe96 Analyzer is a real‑time cell metabolic analyzer that measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96‑well plate format. By quantifying key bioenergetic parameters—mitochondrial respiration, glycolysis, and ATP production—it provides a systems‑level view of cellular metabolism.
Key Features:
Simultaneous OCR and ECAR measurement in 96 wells
Four‑port injection system for timed delivery of substrates and inhibitors
High sensitivity—works with as few as 5,000 cells per well
Precision‑controlled temperature tray (16–42 °C) for consistent assay conditions
Automated mixing and measurement cycles for real‑time kinetic data
Intuitive Wave software for assay design, real‑time monitoring, and advanced data analysis
Applications:
Metabolic phenotyping in cancer, immunology, and stem cell research
Drug screening and toxicity testing
Analysis of genetic or treatment effects on cellular energy production
2D and 3D culture models, including spheroids and organoids
